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Journal of Visualized Experiments (JoVE) is a video journal for biological research. |

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Preparation and Fractionation of Xenopus laevis Egg Extracts Marie K. Cross, Maureen Powers Department of Cell Biology, Emory University
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| Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
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Obtaining Eggs from Xenopus laevis Females Marie K. Cross, Maureen Powers Department of Cell Biology, Emory University
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| The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.
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Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants Cody Locke, Kalen Berry, Bwarenaba Kautu, Kyle Lee, Kim Caldwell, Guy Caldwell Department of Biological Sciences, University of Alabama
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| This video demonstrates how to employ two neural stimulants, aldicarb and pentylenetetrazole (PTZ), in complementary ways to study synaptic function in the nematode, C. elegans. This complementary approach may also be used to shed light on evolutionarily conserved mechanisms for modulating neuronal synchrony and has implications for epilepsy and seizures.
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