All By Issue By Category By Keywords Advanced Search
 

Page  1 2 3 4 5 6 7 8 9 10  

Video Articles

Authors

Keywords
This video demonstrates how to use C. elegans to assess dopaminergic neuron neurodegeneration as a model for Parkinson's disease. Furthermore, genetic screens are used to identify factors that either enhance degeneration or are neuroprotective.
Launch Video

     Published: 07/18/2008
Laura A. Berkowitz
Shusei Hamamichi
Adam L. Knight
Adam J. Harrington
Guy A. Caldwell
Kim A. Caldwell

Department of Biological Sciences, University of Alabama

 
SDD-AGE is a useful technique for the detection and characterization of amyloid-like polymers in cells. Here we demonstrate an adaptation that makes this technique amenable to large-scale applications.
Launch Video

     Published: 07/16/2008
Randal Halfmann1, 2, 3
Susan Lindquist1, 2, 3

1Whitehead Institute for Biomedical Research

2Department of Biology, Massachusetts Institute of Technology

3Howard Hughes Medical Institute

 
In this interview, Dr. Lindquist describes relationships between protein folding, prion diseases and neurodegenerative disorders. The problem of the protein folding is at the core of the modern biology. In addition to their traditional biochemical functions, proteins can mediate transfer of biological information and therefore can be considered a genetic material. This recently discovered function of proteins has important implications for studies of human disorders. Dr. Lindquist also describes current experimental approaches to investigate the mechanism of neurodegenerative diseases based on genetic studies in model organisms.
Launch Video

     Published: 07/16/2008
Susan Lindquist

Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

 
Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.
Launch Video

     Published: 07/11/2008
D. Sesath Hewapathirane1, 2
Kurt Haas1, 2

1Brain Research Centre, University of British Columbia

2Department of Cellular and Physiological Sciences, University of British Columbia

 
Antigen presentation in secondary lymphoid organs by dendritic cells is crucial for the initiation of the T cell mediated adaptive immune response. Here we demonstrate the culture of bone marrow derived murine dendritic cells, activation, and labeling for 2-photon imaging.
Launch Video

     Published: 07/09/2008
Melanie P. Matheu1
Debasish Sen1
Michael D Cahalan 1
Ian Parker 2

1Department of Physiology and Biophysics, University of California, Irvine

2Department of Neurobiology and Behaviour, University of California, Irvine

 
Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.
Launch Video

     Published: 07/08/2008
Sean Gallagher1
Deb Chakavarti2

1Keck Graduate Institute of Applied Life Sciences, UVP, LLC

2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

 
This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina.
Launch Video

     Published: 07/02/2008
Birgit Werner1
Paul B. Cook2, 1
Christopher L. Passaglia3, 1

1Program in Neuroscience, Boston University

2Department of Biology, Boston University

3Department of Biomedical Engineering, Boston University

 
Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.
Launch Video

     Published: 07/02/2008
Richard Ricardo
Katy Phelan

Molecular Pathology Laboratory Network, Inc

 
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
Launch Video

     Published: 06/27/2008
Kelly Morgan1
Michael Kharas1
Elaine Dzierzak2
D. Gary Gilliland1, 3

1Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School

2Department of Cell Biology and Genetics, Erasmus University Medical Center

3Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School

 
We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.
Launch Video

     Published: 06/24/2008
Christos Gekas
Katrin E. Rhodes
Hanna K. A. Mikkola

Jonsson Comprehensive Cancer Center, University of California, Los Angeles

 

 

Page:1 2 3 4 5 6 7 8 9 10 

 

Total: 212 articles.

 

Search results for 7/20/2008 9:07:19 PM

 
 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

Quick links

 

* Welcome  

* Browse  

* Submit  

* About  

* Editorial Board  

* Press  

* Advertising

* Contact  

* Policies

* Article Index

Subscribe
  Subscribe to RSS

You don't have to create an account to receive email notifications. 

Please enter your email:

Subscribe

 

Copyright (C) JoVE 2006-2008 All Rights Reserved.  Powered by TARGET.  JoVE Site Version 1.80